Research Volunteer
DR. NICOLAS CRAPOULET, Ph. D.
Nico Crop
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B.Sc. Biochemistry, Université de Luminy, Marseille, France (1997-2000).

M,Sc. Infectious Diseases and Tropical Pathogens, Faculty of Medicine, Marseille, France, Gene expression analysis of the 5q31-q33 chromosomal region in patients that are resistant or sensitive to infection by Schistosoma mansoni, under the supervision of Christophe Chevillard, Ph.D. (2000-2002).

Ph.D. Infectious Diseases and Tropical Pathogens, Faculty of Medicine, Marseille, France, Post-genomic analysis of Tropheryma whipplei to understand its life cycle, under the supervision of Pr. Didier Raoult (2002-2006).

Postdoctorate, Atlantic Cancer Research Institute, Moncton, NB, Canada. Characterization of isoforms of an essential transcription factor required for B lymphocyte differentiation: Pax5, under the supervision of Dr. Rodney J. Ouellette (2006-2013).


University affiliations:

  • Adjunct professor, Department of Chemistry and Biochemistry, Faculty of Medicine, Université de Sherbrooke (2010-present).
  • Clinical teaching professor, Centre de formation médicale du Nouveau-Brunswick (Moncton) affiliated with Université de Sherbrooke (2010-present).


Research interests

Dr. Crapoulet's research is focused on non-coding RNAs (ncRNAs) and their role in the development of cancer. It has been shown that in higher eukaryotes ncRNAs regulate the expression of genes, help establish chromatin domains, and control the stability of the genome.

An increasing number of studies show that ncRNAs play a central role in the formation of cancer and in cellular differentiation. ncRNAs can be classified into two categories according to their size. Small-interfering RNAs (siRNAs) are well known for their role in the RNA interference pathway and have been well-studied. They control gene expression and the segregation of chromosomes. Large ncRNAs also participate in the functional inactivation of genes and play a key role in cellular differentiation and development. But unlike siRNAs, the mechanisms by which large ncRNAs function are poorly characterized. In order to identify and characterize new ncRNAs in cancer cells, Dr. Crapoulet is using high-throughput screening methods with next-generation sequencing (i.e. RNA-seq, ChIP-seq). Dr. Crapoulet is also using bioinformatics-based approaches to identify ncRNAs in the ENCODE and TCGA databases.

Dr. Crapoulet was responsible for the next-generation sequencing platforms at ACRI and has coordinated the deployment of this technology for molecular clinical diagnostics.

For research purposes, Dr. Crapoulet implemented the next-generation sequencing technology at ACRI in 2012. He has since been working in collaboration with Life Technologies to establish a certified "exome" sequencing facility for ACRI and its collaborators in Atlantic Canada and elsewhere. In partnership with the Dr. G.L. Dumont University Hospital Centre, Dr. Crapoulet has been the principal coordinator for the establishment of a molecular diagnostic platform based on next-generation sequencing. This collaborative service, called the Laboratory for Molecular Diagnostics and Sequencing, was officially opened at the beginning of March 2014 and now offers the possibility to determine the best treatment options for colon and lung cancer patients. Dr. Crapoulet continues to develop and validate new diagnostic methods using next generation sequencing, including the detection of mutations responsible for heritable diseases and the detection of fetal trisomy using maternal blood samples.

 

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